METHOD

Experimental animal model for treatment of absolute uterine factor infertility

Polstyanoy AM1,2, Polstyanaya OYu1,2, Rendashkin IV1, Yakimenko ON1,2, Tutsenko KO1,2, Sadovsky MG1,2,3, Chernova AA1,2
About authors

1 Federal Siberian Research Clinical Center of the Federal Medical Biological Agency, Krasnoyarsk, Russia

2 Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia

3 Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russia

Correspondence should be addressed: Anna A. Chernova
Krasnodarskaya, 40/4, Krasnoyarsk, 660133, Russia; ur.xednay@ksrk-avonrehc

About paper

Funding: the study was conducted as part of the State Assignment of FMBA of Russia.

Author contribution: Polstyanoy AM — developing the surgical protocol for uterus transplantation; Polstyanaya OYu — experimental planning; Rendashkin IV — literature analysis; Yakimenko ON — ethical aspects of study planning; Tutsenko KO — statistical analysis; Sadovsky MG — comparative analysis of experimental data; Chernova AA — management, study planning.

Compliance with the ethical standards: the study was conducted in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Strasbourg, 1986) and the Rules for Handling Experimental Animals (order № 755 of 12.08.1977 of the Ministry of Health of the USSR).

Received: 2022-09-22 Accepted: 2022-12-11 Published online: 2023-01-20
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Reproductive organ transplantation was considered as a potential method for treatment of the ovarian factor, tubal factor, and uterine factor infertility before the advent of advanced assisted reproductive technologies. Uterus transplantation can be considered as the method for treatment of absolute uterine factor infertility similar to transplantation of non-vital organs. However, the clinical use of uterus transplantation in humans causes a lot of problems. The study was aimed to develop a program for assessment of various surgical tissue revascularization techniques for  restoration of reproductive function in experimental animals with uterine factor infertility. Chinchilla rabbits (n = 20) were selected for experiments because of the fact that all mammals have similar structure of the organs. The innovative technique involving the use of ovarian arteries instead of uterine arteries (as in the standard protocol) was used in laboratory animals to develop the surgical protocol for transplantation of reproductive tissues. The animal study results show that hemodynamic characteristics of blood supply to the transplanted uterus remain unchanged. This makes it possible to use the surgical method in the experiments on  the uterus transplant from a deceased donor. The proposed uterus transplantation protocol ensures high transplant survival rate and normal blood supply to the transplant, along with the reduced risk of injury to the donor and reduced complexity of the surgical procedure.

Keywords: uterus transplantation, treatment of uterine factor infertility, organ transplantation, organ donation, comparative analysis

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