2021 / 04

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DOI: 10.47183/mes.2021.037

ORIGINAL RESEARCH

Improvement of the process of production of polysaccharide polyribosyl ribitol phosphate used in the haemophilus influenzae vaccines

Belyankin AA, Salimova EL, Konon AD, Trukhin VP
About authors

St. Petersburg Research Institute of Vaccines and Serums and Bacterial Preparations Production Company, St. Petersburg, Russia

Correspondence should be addressed: Andrey A. Belyankin
Svobody, 52, St. Petersburg, 198320; ur.sviinbps@niknayleb.a.a

About paper

Author contribution: Belyankin AA — collection of information, experimental work and processing of their results; Salimova EL, Konon AD — scientific and technical consulting; Trukhin VP — general management.

Received: 2021-09-15 Accepted: 2021-08-30 Published online: 2021-10-17
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Fig. 1. Growth curves describing cultivation of Haemophilus influenzae type b
Fig. 2. Glucose concentration in cultivation of Haemophilus influenzae type b
Table 1. Impact of aeration on Haemophilus influenzae type b biomass growth and synthesis of the target product during the preparation of inoculum
Note: PRP — polyribosylribitolphosphate; * — the table shows standard deviation.
Table 2. The results of cultivation of Haemophilus influenzae type b in nutrient media containing different sources of protein nitrogen
Note: * — optical density
Table 3. The results of cultivation with various substances acting as X factors
Note: 1 — optical density; 2 — patent composition medium; 3 — medium of patent composition, that contains same concentration of protoporphyrin instead of hemin
Table 4. Comparison of the two Haemophilus influenzae type b cultivation nutrient media by productivity and biomass accumulation
Note: 1 — patent composition medium with meat peptone replaced with soy and pork hemin with protoporphyrin IX.
Table 5. Study of the influence of culture liquid pH of inactivation