Biomedical support is aimed at provision of the athletes' training at various stages of the training and competition process. Withholding of access to this process due to health problems resulting from hepatitis virus infection is a demanding task. The study was aimed to assess the detection rate of the hepatitis B virus, hepatitis C virus and TT virus infection markers in highly trained athletes. A total of 384 blood serum samples were collected from 240 males and 144 females aged 14–49 (athletes engaged in playing sports, precision sports, technical sports, etc.) within the framework of the multicenter open-label cross-sectional clinical trial. All athletes answered a questionnaire, which included demographic information, characteristics of sports, information about the infection risk factors, information about the fact of  past acute viral hepatitis and vaccination. Markers of infection with hepatitis B virus, hepatitis C virus and TTV were identified in blood serum by enzyme immunoassay. HbsAg was detected in two surveyed athletes. Anti-HBcore (surrogate marker of latent HBV infection) was detected in 7% of samples (27/384); 1% of athletes (4/384) had a positive hepatitis C virus total antibody test (anti-HCV). Аnti-HCV in combination with anti-HBcore was detected in one female athlete (14 years of age, tennis player). DNA of TTV, TTMDV and TTMV was detected in blood serum samples of 89.1%, 83.1% and 85.4% of athletes, respectively. High detection rate of the hepatitis virus markers was observed.