Increased workloads among heads of clinical departments that result from working as both clinicists and managers may lead to the significant decline in their psychological well-being. The study was aimed to assess psychological well-being of the clinical department heads. The online survey of 216 department heads aged 32–70 having a 8–51-year experience in heathcare was conducted using the Ryff's scales of psychological well-being adopted by Shevelenkova–Fesenko, sent by e-mail or posted on the distance learning portal. The survey involved 123 men (56.9%) and 93 women (43.1%%): among them 117 people (54.2%) worked in inpatient settings, 114 people (52.8%) worked in the red zone, 138 people (63.89%) were assigned a qualification category, 63 people (29.1%) had an academic degree. Mean values, percentage, Pearson correlation coefficient, Student's t-test were calculated with the IBM SPSS Statistics 23 software. Correlations and mean differences were considered significant at р < 0.05. The average psychological well-being score was 378.67 ± 78.33; in 26 men (26%) and 28 women (43.1%) aged 36–55, the score was below standard values. Psychological well-being correlated with age (r = 0.2; p = 0.019) and years of service (r = 0.2; p = 0.008). No correlations were revealed between psychological well-being and gender (р = 0.798), type of organization (р = 0.642), the fact of having second higher education (р = 0.854), qualification category (р = 0.645), academic degree (р = 0.204), and the experience of working in the red zone (р = 0.926).Thus, more than a third of individuals aged 36–55 have psychological well-being scores below standard values. Psychological well-being of women is lower than that of men. Psychological well-being of men over the age of 35 decreases to a greater extent than standard values.