ISSN Print 2713-2757
ISSN Online 2713-2765
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Effect of sodium bicarbonate on the development of gastric stasis in the rat model of myeloablative chemotherapy with cyclophosphamide
About authors
1 Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency, Saint-Petersburg, Russia
2 State Scientific Research Test Institute of the Military Medicine of Defense Ministry of the Russian Federation, Saint-Petersburg, Russia
Correspondence should be addressed: Timur V. Schäfer
Lesoparkovaya, 4, Saint-Petersburg, 195043, Russia; ur.xednay@refahcs
About paper
Author contribution: Vakunenkova OA — experimental study; Ivnitsky JuJu — rationale, developing the experimental model, data interpretation and discussion; Gaykova ON — morphometry data interpretation; Kozlov AA — morphometry studies; Schäfer TV — experimental procedure, data processing and visualization, developing the experimental model. All authors contributed to discussion, manuscript writing and editing.
Compliance with ethical standards: the study was carried out in accordance with the principles of bioethics, approved by the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes.
Received: 2023-04-06
Accepted: 2023-05-19
Published online: 2023-06-12
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Fig. 1. Abdominal organs of the rats deprived of food 48 h before laparotomy: intact (А); 72 h after intravenous injection of cyclophosphamide in a dose of 390 mg/kg (B). Arrows show: 1 — stomach; 2 — caecum
Fig. 2. Relative weight of the gastric (А) and caecal (B) chyme in rats 72 h after intravenous injection of cyclophosphamide in a dose of 390 mg/kg (M ± m; n = 10) depending on the time of access to food after the exposure. Controls — animals not administered with cyclophosphamide. At the beginning of the horizontal axis — values of the group of rats not administered with cyclophosphamide and having unlimited access to food. * — significant differences from controls (p < 0.05)
Fig. 3. Relative weight of the gastric chyme, caecal chyme and the spleen in rats 72 h after intravenous injection of cyclophosphamide in a dose of 390 mg/kg (M ± m; n = 10). “Intact” — rats not administered with drugs; “CP” — rats administered with cyclophosphamide only; “SB + CP” — rats that received intragastric administration of the 4% sodium bicarbonate solution 30 before the cyclophosphamide administration; “SB + CP + SB” — rats that received intragastric administration of the 4% sodium bicarbonate solution 30 before and immediately after the cyclophosphamide administration; “SB + CP + SB”— rats that received intragastric administration of the 4% sodium bicarbonate solution 30 before, immediately after, 1 and 2 h after the cyclophosphamide administration. All the animals were deprived of food 24 h after the cyclophosphamide administration. Significant differences (p < 0.05): * — from the intact group; † — from the “CP” and “NaHCO3 + CP” groups
Fig. 4. Urinary excretion of indican in rats 72 h after intravenous injection of cyclophosphamide in a dose of 390 mg/kg (M ± m; n = 10). The symbols are the same as in Fig. 3
Fig. 5. Annular slices of the duodenum, jejunum, and ileum of the rats 72 h after intravenous injection of cyclophosphamide in a dose of 390 mg/kg
Table. Morphological signs of acute chemotherapy-induced gastrointestinal mucositis in rats 72 h after intravenous injection of cyclophosphamide in a dose of 390 mg/kg
Note: “Intact” — rats that received no drug treatment; “CP” — rats that received cyclophosphamide only; “NaHCO3 + CP + NaHCO3 ” — intragastric administration by oral gavage of 4% sodium bicarbonate solution 10 min before and immediately after the cyclophosphamide administration. Significant differences (p < 0.05): * — from the intact group; † — from the “CP” group.
