2023 / 04

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DOI: 10.47183/mes.2023.051

ORIGINAL RESEARCH

Methylation of cell cycle and apoptosis genes’ promoters in exposed individuals with subsequent malignant neoplasms

Blinova EA1,2, Korechenkova AV1, Nikiforov VS1,2, Akleyev AV1,2
About authors

1 Urals Research Center for Radiation Medicine, Chelyabinsk, Russia

2 Chelyabinsk State University, Chelyabinsk, Russia

Correspondence should be addressed: Evgenia A. Blinova
Vorovskogo, 68A, Chelyabinsk, 454141, Russia; ur.mrcru@avonilb

About paper

Acknowledgements: the article was prepared in the context of the Federal Target Program "Modernization of high-tech methods of identification of medical consequences of exposure to radiation of personnel of the Mayak Production Association and population of the Ural region," Contract № 27.501.21.2 of 11.06.2021.

Author contributions: E.A. Blinova — study planning, generalization of primary material, analysis and discussion of the results, article drafting; A.V. Korechenkova — laboratory tests, article drafting; V.S. Nikiforov — laboratory tests, article drafting; A.V. Akleyev — study planning, article editing, authoring of the final version of the article.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Urals Research Center for Radiation Medicine of the FMBA of Russia (Minutes #2 of July 20, 2021). All participants signed the informed consent form to participate in the study.

Received: 2023-10-03 Accepted: 2023-11-13 Published online: 2023-12-25
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Fig. Distribution of the participants by level of methylation of CpG-dinucleotides of the studied gene promoter regions. Chi-squared test value incorporates Yates correction
Table 1. Characteristics of the studied groups
Note: RBM — red bone marrow, M — mean; SE — standard error; n — number of people; (min–max) — range of values.
Table 2. Characteristics of the used oligonucleotides
Note: F — forward primer; R — reverse primer; Ta — annealing temperature.
Table 3. Cases of methylation of CpG islands of the promoter regions of BAX, MDM2, TP53, NFkB1 in the study sample
Note: р — is the level of statistical significance of differences between groups, as given by Fisher's exact test.
Table 4. Spearman's rank correlation coefficients (R), dependence of the degree of methylation of the studied gene promoter regions on RBM dose and patient's age at the time of the study. The p-value for Spearman's correlation coefficients is given in parentheses