ISSN Print 2713-2757
ISSN Online 2713-2765
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
Copyright: © 2021 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Isolation and characterization of Pseudomonas aeruginosa bacteriophages — potential agents for phage therapy
About authors
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
Correspondence should be addressed: Maria A. Kornienko
Malaya Pirogovskaya, 1a, Moscow, 119435; moc.liamg@ayiramokneinrok
About paper
Funding: The study was supported by the State Assignment on the Development of a personalized approach to the therapy of infections using virulent bacteriophages (Code: Bacteriophage) (Russia).
Acknowledgments: the authors thank the Center for Precision Genome Editing and Genetic Technologies for Biomedicine, the Federal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical Biological Agency for their help with sequencing the genomes of bacteriophages.
Author contribution: Kornienko MA — study plan, data collection and processing, article authoring; Kuptsov NS — data collection and processing, article authoring; Danilov DI, Gorodnichev RB, Malakhova MV, Veselovsky VA — data collection; Bespiatykh DA — data processing, Shitikov EA — research plan, data processing, article authoring; Ilina EN — research plan, article authoring.
Compliance with ethical standards: the experiment was carried out in compliance with the Sanitary and Epidemiological Rules SP 1.3.2322-08 "Safe work with microorganisms of III–IV pathogenicity (hazardousness) groups and pathogens of parasitic diseases"; Sanitary and Epidemiological Rules SP 1.3.2518-09 "Amendments and additions #1 to the sanitary and epidemiological rules" Safe work with microorganisms of III–IV pathogenicity (hazardousness) groups and pathogens of parasitic diseases"; Sanitary and Epidemiological Rules SanPiN 2.1.7.2790-10 "Sanitary and epidemiological requirements for medical waste management"; Federal Clinical Recommendations "Rational use of bacteriophages in medical and anti-epidemic practice."
Received: 2021-07-19
Accepted: 2021-08-05
Published online: 2021-09-18
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Fig. 1. Phylogenetic analysis of the whole genome sequences of Pseudomonas spp bacteriophages A. Phylogenetic tree of the Autographiviridae family bacteriophages (n = 50). B. Phylogenetic tree of the Myoviridae family bacteriophages (n = 60). Bacteriophages vB_PaeA-55-1w and vB_PaeM-198 are marked in red
Fig. 2. Main structural modules of the genomes of vB_PaeA-55-1w and vB_PaeM-198 P. aeruginosa bacteriophage
Table 1. Characteristics of P. aeruginosa bacteriophage host strains
Table 2. Genetic characteristics of the collection strains and range of the hosts of vB_PaeA-55-1w and vB_PaeM-198 bacteriophages
Note: * — the allele numbers of genes included in the standard MLST pattern (arcC-aroE-glpF-gmk-pta-tpi-yqi) [21] are indicated for each unique sequence type.
