Antimicrobial and antiviral activity of three-component complex of chlorhexidine-edta-zinc

Galinkin VA1, Enikeev AKh1, Podolskaya EP2,3, Gladchuk AS2,4, Vinogradova TI5, Zabolotnykh NV5, Dogonadze MZ5, Krasnov KA2
About authors

1 OOO ”ROSBIO”, St. Petersburg, Russia

2 Federal State-Financed Institution Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency, St. Petersburg, Russia

3 Institute of Analytical Instrumentation, St. Petersburg, Russia

4 Saint Petersburg State University, St Petersburg, Russia

5 Saint-Petersburg State Research Institute of Phtisiopulmonology, St. Petersburg, Russia

Correspondence should be addressed: Konstantin A. Krasnov
Bekhtereva, 1/2, k. 54, St. Petersburg, 199106, Russia; ur.liam@xot_vonsark

About paper

Funding: ООО "ROSBIO"

Author contribution: Galinkin VA — organization of microbiological and virological research; Enikeev AKh — general management; Podolskaya EP — mass spectrometric analysis, Gladchuk AS — chemical-analytical studies; Vinogradova TI — management of studies of anti-tuberculosis activity; Zabolotnykh NV — study of antitubercular activity in vitro; Dogonadze MZ — study of antitubercular activity in animals; Krasnov KA — data processing and interpretation, preparation of the materials for publication.

Compliance with ethical standards: all procedures with animal models were carried out in accordance with the Directive 2010/63/EU of the European Parliament and of the Council of the European Union (2010) on protection of animals used for scientific purposes.

Received: 2021-12-27 Accepted: 2022-01-24 Published online: 2022-02-07

Chlorhexidine bigluconate (CНX) is widely used as a disinfectant, but it is not effective against spore-forming microorganisms, as well as viruses. In this work, a method has been found to increase the biocidal activity of chlorhexidine by using it as part of a complex including ethylenediaminetetraacetic acid (EDTA) and zinc chloride. The structure of the three-component complex СНX-EDTA-zinc is proved by the MALDI-MS method. The biocidal activity of the chlorhexidine complex has been studied in vitro and in vivo experiments. It is shown that the complex is significantly superior to chlorhexidine alone, both in terms of activity level and in the breadth of biocidal action. In relation to the studied bacterial and fungal strains, the СНX-EDTA-Zn complex was 4–5 times more active than chlorhexidine bigluconate. In concentrations from 1.0 mg/ml to 0.008 mg/ml (depending on the type of micro-organism), in vitro the complex showed both bacteriostatic and bactericidal effects against the main pathogens of bacterial diseases of birds. In clinical conditions, the complex has shown high efficiency in the treatment of dermatitis in small domestic and farm animals. Also, in vitro and in vivo, the complex showed unexpectedly high antitubercular activity comparable to that of monofloxacin, including on drug-resistant strains of mycobacteria. in vitro experiments involving polio virus and adenovirus have shown that the СHX-EDTA-Zn complex possesses virulent action.

Keywords: antiviral activity, antiseptic, chlorhexidine, EDTA, zinc, complex, MALDI, anti-tuberculosis