2022 / 01

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DOI: 10.47183/mes.2022.002

ORIGINAL RESEARCH

Antimicrobial and antiviral activity of three-component complex of chlorhexidine-edta-zinc

Galinkin VA1, Enikeev AKh1, Podolskaya EP2,3, Gladchuk AS2,4, Vinogradova TI5, Zabolotnykh NV5, Dogonadze MZ5, Krasnov KA2
About authors

1 OOO ”ROSBIO”, St. Petersburg, Russia

2 Federal State-Financed Institution Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency, St. Petersburg, Russia

3 Institute of Analytical Instrumentation, St. Petersburg, Russia

4 Saint Petersburg State University, St Petersburg, Russia

5 Saint-Petersburg State Research Institute of Phtisiopulmonology, St. Petersburg, Russia

Correspondence should be addressed: Konstantin A. Krasnov
Bekhtereva, 1/2, k. 54, St. Petersburg, 199106, Russia; ur.liam@xot_vonsark

About paper

Funding: ООО "ROSBIO"

Author contribution: Galinkin VA — organization of microbiological and virological research; Enikeev AKh — general management; Podolskaya EP — mass spectrometric analysis, Gladchuk AS — chemical-analytical studies; Vinogradova TI — management of studies of anti-tuberculosis activity; Zabolotnykh NV — study of antitubercular activity in vitro; Dogonadze MZ — study of antitubercular activity in animals; Krasnov KA — data processing and interpretation, preparation of the materials for publication.

Compliance with ethical standards: all procedures with animal models were carried out in accordance with the Directive 2010/63/EU of the European Parliament and of the Council of the European Union (2010) on protection of animals used for scientific purposes.

Received: 2021-12-27 Accepted: 2022-01-24 Published online: 2022-02-07
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Fig. 1. Changes in the intensity of growth of the M. tuberculosis H37Rv test strain under the influence of the CHX-EDTA-Zn complex. The abscissa shows the drug concentration, the ordinate shows the average fluorescence intensity in conventional units, M is the mean value, (M-I) and (M+I) are the lower and upper limits of the confidence interval (at α = 0.05)
Fig. 2. Structural formula of the chlorhexidine-EDTA-zinc complex
Table 1. Antimicrobial activity of the CHX-EDTA-Zn complex in comparison with chlorhexidine bigluconate (CHX)
Table 2. CHX-EDTA-Zn complex activity against avian pathogens
Note: «–» — complete absence of growth; "+" — growth of single colonies; "+" — normal growth.
Table 3. CHX-EDTA-Zn complex efficacy indicators in treatment of dermatitis in animals
Table 4. Tuberculosis infection severity in mice infected with M. tuberculosis H37Rv and infected with drug-resistant strain 5582 (after 5 weeks from the start of the treatment)
Table 5. Inoculation of MBT from the lungs of mice infected with M. tuberculosis H37R and strain 5582 after 5 weeks of treatment with the CHX-EDTA-Zn complex
Table 6. Virucidal activity of the CHX-EDTA-Zn complex