ISSN Print 2713-2757
ISSN Online 2713-2765
Extreme Medicine
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
METHOD
Development of PCR test for detection of the SARS-CoV-2 genetic variants alpha, beta, gamma, delta
About authors
Centre for Strategic Planning and Management of Biomedical Health Risks of Federal Medical Biological Agency, Moscow, Russia
Correspondence should be addressed: Anna K. Shuryaeva
Pogodinskaya, 10, str. 1, 119121, Moscow; ur.zmpsc@aveayruhsa
About paper
Author contribution: Shipulin GA, Savochkina YuA, Davydova EE, Yudin SM — planning the experiment; Shipulin GA, Savochkina YuA, Yudin SM — literature analysis; Shuryaeva AK, Shivlyagina EE, Nosova AO, Luparev AR, Malova TV — experimental procedure, data interpretation; Savochkina YuA, Shivlyagina EE, Nosova AO — reagent kit development; Luparev AR — statistical analysis; Shipulin GA, Shuryaeva AK — manuscript writing and editing; Davydova EE, Yudin SM — manuscript editing.
Compliance with ethical standards: the study was performed in accordance with the requirements of the Declaration of Helsinki and GOST R ISO 14155-2014.
Received: 2021-12-15
Accepted: 2022-01-13
Published online: 2022-02-09
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Figure. The results for the SARS-CoV-2 VOC spread monitoring performed from December 2020 to September 2021. The samples (total n = 10,297) were collected in Moscow and Moscow Region
Table. The RNA targets with corresponding fluorescent probes to be detected for each RT-PCR mixture of the kit
