2022 / 03

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DOI: 10.47183/mes.2022.027

ORIGINAL RESEARCH

Transcriptional activity of DNA-methyltransferase genes in the chronically exposed residents of the Ural region

Nikiforov VS1,2, Blinova EA1,2, Akleyev AV1,2
About authors

1 Urals Research Center for Radiation Medicine of the Federal Medical Biological Agency, Chelyabinsk, Russia

2 Chelyabinsk State University, Chelyabinsk, Russia

Correspondence should be addressed: Vladislav S. Nikiforov
Vorovskogo, 68, korp. 1, Chelyabinsk, 454141, Russia; ur.liam@xvorofikin

About paper

Funding: the study was supported by the Federal Medical Biological Agency of Russia (Government contract № 27.501.21.2 of June, 11 2021).

Author contribution: Nikiforov VS — laboratory tests, statistical analysis, manuscript writing; Blinova EA, Akleyev AV— study planning, manuscript editing and preparing the final version of the article.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Urals Research Center for Radiation Medicine of FMBA of Russia (protocol № 3 dated June 28, 2022). The informed consent was submitted by all patients.

Received: 2022-06-29 Accepted: 2022-07-17 Published online: 2022-08-09
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Fig. Relative mRNA levels of DNMT1 (RU) in exposed individuals as a function of dose to RBM (А), thymus and peripheral lymphoid organs (B): the dotted line indicates linear approximation (trend line); # indicates significant differences in DNMT1 gene expression between the comparison group and the group of chronically exposed people; vertical error bar corresponds to the error of the mean of the relative mRNA levels of DNMT1; horizontal error bar corresponds to the error of the mean of the accumulated dose to RBM (А), thymus and peripheral lymphoid organs (B)
Table 1. Olygonucleotide sequences of primers and probes
Table 2. Relative mRNA levels (Ме) of DNA methyltransferase genes (RU) in peripheral blood cells of chronically exposed people over a long-term period
Note: in parentheses are 25th–75th percentile; n — sample size.
Table 3. Spearman's rank correlation (R) between the relative mRNA levels of DNA methyltransferase genes and the values of dose and dose rate during the period of the highest levels of radiation exposure