ORIGINAL RESEARCH
Transcriptional activity of DNA-methyltransferase genes in the chronically exposed residents of the Ural region
1 Urals Research Center for Radiation Medicine of the Federal Medical Biological Agency, Chelyabinsk, Russia
2 Chelyabinsk State University, Chelyabinsk, Russia
Correspondence should be addressed: Vladislav S. Nikiforov
Vorovskogo, 68, korp. 1, Chelyabinsk, 454141, Russia; ur.liam@xvorofikin
Funding: the study was supported by the Federal Medical Biological Agency of Russia (Government contract № 27.501.21.2 of June, 11 2021).
Author contribution: Nikiforov VS — laboratory tests, statistical analysis, manuscript writing; Blinova EA, Akleyev AV— study planning, manuscript editing and preparing the final version of the article.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Urals Research Center for Radiation Medicine of FMBA of Russia (protocol № 3 dated June 28, 2022). The informed consent was submitted by all patients.
- Arand J, Spieler D, Karius T, Branco MR, Meilinger D, Meissner A, et al. In vivo control of CpG and non-CpG DNA methylation by DNA methyltransferases. PLOS Genetics. 2012; (8): 1002750. DOI:10.1371/journal.pgen.1002750.
- Jeltsch A, Jurkowska RZ. New concepts in DNA methylation. Trends in Biochemical Sciences. 2014; 39 (7): 310–18. DOI: 10.1016/j.tibs.2014.05.002.
- Chaudhry MA, Omaruddin RA. Differential DNA methylation alterations in radiation-sensitive and -resistant cells. DNA and Cell Biology. 2012; 31 (6): 908–16. DOI: 10.1089/dna.2011.1509.
- López-Nieva P, González-Vasconcellos I, González-Sánchez L, Cobos-Fernández MA, Cobos-Fernández S, Pérez RS, et al. Differential molecular response in mice and human thymocytes exposed to a combined-dose radiation regime. Sci Rep. 2022; (12): 3144. DOI: 10.1038/s41598-022-07166-8.
- Chen B, Dai Q, Zhang Q , Yan P, Wang A, Qu L, et al. The relationship among occupational irradiation, DNA methylation status, and oxidative damage in interventional physicians. Medicine (Baltimore). 2022; 98 (39): e17373. DOI: 10.1097/ MD.0000000000017373.
- Peterson EJ, Bogler O, Taylor SM. p53-mediated repression of DNA methyltransferase 1 expression by specific DNA binding. Cancer Res. 2003; 63 (20): 6579–82.
- Armstrong CA, Jones GD, Anderson R, Iyer P, Narayanan D, Sandhu J, et al. DNMTs are required for delayed genome instability caused by radiation. Epigenetics. 2012; 7 (8): P. 892–902. DOI: 10.4161/epi.21094.
- Rugo RE, Mutamba JT, Mohan KN, Yee T, Greenberger JS, Engelward BP. Methyltransferases mediate cell memory of a genotoxic insult. Oncogene. 2011; 30 (6): 751–6. DOI: 10.1038/ onc.2010.480.
- Degteva MO, Napier BA, Tolstykh EI, Shishkina EA, Bougrov NG, Krestinina LYu, et al. Individual dose distribution in cohort of people exposed as a result of radioactive contamination of the Techa River. Мedical Radiology and Radiation Safety. 2019; 64 (3): 46–53. DOI: 10.12737/article_5cf2364cb49523. 98590475.
- Degteva MO, Napier BA, Tolstykh EI, Shishkina EA, Shagina NB, Volchkova AYu, et al. Enhancements in the Techa River Dosimetry System: TRDS-2016D code for reconstruction of deterministic estimates of dose from environmental exposures. Health Physics. 2019; 117 (4): 378–87. DOI: 10.1097/HP.0000000000001067.
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods. 2001; 25 (4): 402–8. DOI: 10.1006/meth.2001.1262.
- Svedruzic ZM. Dnmt1 structure and function. Prog Mol Biol Transl Sci. 2011; 101: 221–54. DOI: 10.1016/B978-0-12-387685-0.00006-8.
- Lee Y, Kim YJ, Choi YJ, Lee JW, Lee S, Cho YH, et al. Radiation-induced changes in DNA methylation and their relationship to chromosome aberrations in nuclear power plant workers. Int J Radiat Biol. 2015; 91 (2): 142–9. DOI: 10.3109/09553002.2015.969847.
- Cho YH, Jang Y, Woo HD, Kim YJ, Kim SY, Christensen S, et al. LINE-1 hypomethylation is associated with radiation-induced genomic instability in industrial radiographers. Environ Mol Mutagen. 2018; 60 (2):174–84. DOI: 10.1002/em.22237.
- Kuzmina NS, Lapteva NSh, Rubanovich AB. Hypermethylation of gene promoters in peripheral blood leukocytes in humans long term after radiation exposure. Environ Res. 2016; 146: 10–17. DOI: 10.1016/j.envres.2015.12.008.
- Kuzmina NS, Lapteva NSh, Rusinova GG, Azizova TV, Vyazovskaya NS, Rubanovich AV. Dose dependence of hypermethylation of gene promoters in blood leukocytes in humans exposed to both gamma-radiation and alpha-particles. Genetika. 2018; 54: S22– 26. DOI: 10.1134/S0016675818130118.
- Blinova EA, Nikiforov VS, Kotikova AI, Yanishevskaya MA, Akleyev AV. Methylation status of apoptose genes and intensity of apoptotic death of peripheral blood lymphocytes in persons chronically exposed to radiation. Molecular Biology. 2022; 56 (5): 1–11.
- Nikiforov VS, Blinova EA, Akleyev AV. The transcriptional activity of cell cycle and apoptosis genes in chronically exposed people with an increased frequency of TCR mutant lymphocytes. Radiation and Risk (Bulletin of the National Radiation and Epidemiological Registry). 2020; 29 (2): 89–100. DOI: 10.21870/0131-38782020-29-2-89-100.