ORIGINAL RESEARCH

Development of microbial preparation for bioremediation of soils contaminated with rocket fuel components

Zharikov GA1, Krainova OA1, Khaitov MR2, Marchenko AI1
About authors

1 Research Center for Toxicology and Hygienic Regulation of Biopreparations of the Federal Medical Biological Agency, Bolshevik, Moscow region, Russia

2 National Research Center Institute of Immunology of the Federal Medical Biological Agency, Moscow, Russia

Correspondence should be addressed: Gennady A. Zharikov
Lenina, 102А, Bolshevik, Serpukhov city district, Moscow region, 142253, Russia; ur.oibcixot@vokirahZ

About paper

Funding: the study was carried out under state orders of the Federal Medical Biological Agency of Russia (№ 26.008.02.0, № 22.009.21.800).

Author contribution: Khaitov MR — general research management; Zharikov GA — planning and management of laboratory and field research, experimental procedure, data analysis; Krainova OA — microbiological testing (isolation and selection of bacterial destructors of rocket fuel, preparation of microbial suspensions for experiments, biodestructor collection maintenance); Marchenko AI — microbiological and biochemical testing (enzyme activity of soil), biotests for assessment of soil toxicity, statistical data processing.

Compliance with ethical standards: animals were treated in accordance with the principles of Good Laboratory Practice. Veterinary protocols № 669 and № 677 for strains 5G и 62М/3 were approved by the Bioethics Commission (protocol № 165/2019 of 19 February 2019, protocol № 169/2019 of 16 April 2019).

Received: 2022-06-22 Accepted: 2022-08-18 Published online: 2022-09-15
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  1. Rossijskij registr potencial'no opasnyh himicheskih i biologicheskih veshhestv. 1,1-dimetilgidrazin. Registracionnyj nomer VT002431. Available from: https://www.rpohv.ru/online/detail. html?id=2431. Russian.
  2. Petrova ZM, Ostapenko NS, Bojcova LV. Migracija nesimmetrichnogo dimetilgidrazina i ego proizvodnyh pri rekul'tivacii zagrjaznennyh pochv. Pochvovedenie. 1999; 12: 1502–8. Russian.
  3. Pimkin VG, Kachin VG. Metody i sredstva lokalizacii i obezvrezhivanija KRT v okruzhajushhej srede. SPb.: Izd. RNC prikladnoj himii, 1992; 184 p. Russian.
  4. Emeljanova EK, Alekseev AJu, Mokeeva AV. Biorekul'tivacija zagrjaznennyh neftju objektov v Tjumenskoj oblasti. Vestnik Novosibirskogo gosudarstvennogo universiteta. Serija: Biologija, klinicheskaja medicina. 2010; 8 (4): 155–61. Russian.
  5. Malyhina LV, Shajdullina IA, Antonov NA. Primenenie biopreparata “Gumaks” i aborigennyh shtammov mikroorganizmov– destruktorov nefti v sochetanii s nanobentonitom dlja rekul'tivacii tipichnyh i vyshhelochennyh chernozemov so smeshannym tipom neftezagrjaznenija. Sbornik nauchnyh trudov TatNIPIneft' PAO «Tatneft'». M., 2016; p 298–307. Russian.
  6. Oliskevich VV, Talalovskaja NM, Tretjakova SJ. Optimizacija tehnologii bioremediacii sel'skohozjajstvennyh zemel', zagrjaznennyh gerbicidom “Gezagard”. Izvestija Saratovskogo universiteta. Serija: Himija. Biologija. Jekologija. 2013; 13 (2): 101–7. Russian.
  7. Chetverikov SP. Mikrobiologicheskie metody rekul'tivacii neftezagrjaznennyh zemel'. Ural'skij jekologicheskij vestnik. 2012; 4 (33): 34–35. Russian.
  8. Pimkin VG, Sulima TA, Sofin AP. Dezaktivacija pochv posle avarijnyh i tehnologicheskih prolivov NDMG V sbornike: Jekologicheskie aspekty vozdejstvija komponentov zhidkih raketnyh topliv na okruzhajushhuju sredu. Materialy nauchnoprakticheskoj konferencii. SPb.: Izd-vo RNC prikladnoj himii, 1996; p. 91–104.
  9. Tehnologicheskij reglament na detoksikaciju pochv, zagrjaznennyh nesimmetrichnym dimetilgidrazinom i produktami ego himicheskoj transformacii kombinirovannym metodom. Nacional'noe kosmicheskoe agentstvo respubliki Kazahstan. Almaty, 2012; 18 p.
  10. Sposob biodestrukcii geptila. Patent RF № 98102248A. 11.02.1998.
  11. Zabokrickij AA, Savinyh DJu, Tarabara AV, Zorin AD, Zabokrickij NA, Zanozina VF i dr. Biologicheskij destruktor nesimmetrichnogo dimetilgidrazina. Patent RF № 2650864. 17.04.2018.
  12. Zvjagincev DG, editor. Metody pochvennoj mikrobiologii i biohimii. M.: Izd-vo MGU, 1991; 304 p. Russian.
  13. Zharikov GA, Krajnova OA, Kapranov VV, Djadishheva VP, Kiseleva NI. Shtamm bakterij Rhodococcus globerulus 19F, razlagajushhij 1,1-dimetilgidrazin (geptil). Patent RF № 2236453. 18.09.2002.
  14. Metodika opredelenija toksichnosti vodnyh vytjazhek iz pochv, osadkov stochnyh vod i othodov, pit'evoj, stochnoj i prirodnoj vody po smertnosti test-objekta Daphnia magna Straus. PND F T 14.1:2:3:4.12-06. Izdanie 2021 g. 16.1:2:2.2:2.3:3.9-06 FR.1.39.2021.40207; 15 p. Russian.
  15. Metodika opredelenija toksichnosti vody i vodnyh vytjazhek iz pochv, osadkov stochnyh vod, othodov po smertnosti i izmeneniju plodovitosti dafnij. FR.1.39.2001.00283; 34 p. Russian.
  16. Metodika opredelenija integral'noj toksichnosti poverhnostnyh, v tom chisle morskih, gruntovyh, pit'evyh, stochnyh vod vodnyh jekstraktov pochv, othodov, osadkov stochnyh vod po izmeneniju intensivnosti bakterial'noj bioljuminescencii test-sistemoj “Jekoljum”. PND F T 14.1:2:3:4.11-04 T 16.1:2.3:3.8-04. M.: Ministerstvo prirodnyh resursov Rossijskoj Federacii, 2010; 22 p. Russian.
  17. Metodika opredelenija toksichnosti himicheskih veshhestv, polimerov, materialov i izdelij s pomoshh'ju biotesta “Jekoljum”. Metodicheskie rekomendacii N 01.018-07. M.: Federal'nyj centr Gossanjepidnadzora Minzdrava Rossii, 2014; 14 p. Russian.
  18. Mineev VG, editor. Praktikum po agrohimii. 2 izd., pererab. i dop. M.: Izd-vo MGU, 2001; 689 p. Russian.
  19. Beresteckij OA. Metody opredelenija toksichnosti pochvy. Mikrobiologicheskie i biohimicheskie issledovanija pochv. Kiev: Izd-vo “Urozhaj”, 1971; 208 p. Russian.
  20. Metodika izmerenij massovoj koncentracii nefteproduktov v pit'evyh, poverhnostnyh i stochnyh vodah metodom IKspektrometrii. PND F 14.1:2:4.5-95. M.: Ministerstvo prirodnyh resursov Rossijskoj Federacii; 2011. 18 p. Russian.
  21. Metodicheskie ukazanija po gigienicheskoj ocenke mikrobiologicheskih sredstv zashhity rastenij ot nasekomyh i boleznej na osnove nesporoobrazujushhih mikroorganizmov (MU № 2620-82).1982; 24 p. Russian.
  22. Metodicheskie ukazanija po jeksperimental'nomu obosnovaniju PDK mikroorganizmov-producentov i soderzhashhih ih gotovyh form preparatov v ob#ektah proizvodstvennoj i okruzhajushhej sredy (MU № 5789/1-91). 1991; 23 p. Russian.
  23. Sheina NI. Kriterii ocenki patogennyh svojstv shtammov-producentov, predlagaemyh dlja ispol'zovanija v promyshlennosti mikrobiologicheskogo sinteza. Vestnik OGU. 2012; 6 (142): 165–9.