2022 / 04

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DOI: 10.47183/mes.2022.046

ORIGINAL RESEARCH

Immunochromatography-based portable equipment for indication of pathogenic microorganisms and toxins

Yarkov SP, Shilenko IV, Tretyakov SI, Ishkov YuN, Styazhkin KK
About authors

Federal State Unitary Enterprise "State Scientific Research Institute of Biological Engineering" FMBA of Russia (FSUE “SSRIBE”), Moscow, Russia

Corresponding author: Sergey P. Yarkov
Volokolamskoye, 75, str. 1, 125424, Moscow, Russia; ur.lod@losaid

About paper

Funding: the studies were carried out under government contracts with the Russian Federal Medical and Biological Agency (No. 42.128.11.6, No. 42.133.12.6) and the Russian Ministry of Health (No. K-27-FTP/82-1).

Contributing authors: Yarkov SP ― idea, development planning, analysis of findings, participation in tests, manuscript preparation; Shilenko IV ― creation of multi-analysis lateral flow immunoassays, implementation of tests of EkB sets, analysis of obtained results; Tretyakov SI ― creation of lateral flow immunoassays, models and kits, tests, analysis of research results; Ishkov YN ― study management, manuscript editing; Stiazhkin KK ― management, manuscript editing.

Received: 2022-11-26 Accepted: 2022-12-18 Published online: 2022-12-29
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Fig. 1. Schematics of the test strip for the LFIA. 1 — rigid plastic substrate; 2 — substrate for sample application; 3 — conjugate substrate with dried conjugate of colloidal gold nanoparticles (CGN) with specific antibodies; 4 — analytical membrane with applied lines of antibody solutions; 6 — analytical zone; 7 — control zone; 5 — adsorbent substrate. The vertical arrow indicates the application of the liquid sample on the substrate, the horizontal arrow indicates the direction of flow of the test sample
Fig. 2. Appearance of the multi-analyte lateral flow immunoassays included in the EkB and EkB-01 kits. А. Bacteria for multi-analyte lateral flow immunoassays. B. Viruses and accumulation media for multi-analyte lateral flow immunoassays. C. Toxins for multi-analyte lateral flow immunoassays 1 — sample put-in opening; 2 — opening for recording the test finding; 3 — analytical area; 4 — control area
Fig. 3. Exterior view of the reflectometer device of EkB kit
Fig. 4. Exterior view of the EkB-01 kit. A. Kit with open cover. B. Attachment of the kit to the operator's uniform
Table 1. Comparative characteristics of domestic and foreign technical tools for the indication of pathogens in environmental media based on lateral flow immunoassays
Note: * — the decimal numbers of the design documentation are indicated; * * — lateral flow immunoassays use a fluorescent tag; * * * — SEB — Staphylococcal enterotoxin type B; The table contains manufacturers' information on indication equipment such as portable kits and sets to counteract the bioterrorist threat. The list of individual lateral flow immunoassays produced in Russia and abroad for medical purposes for the diagnosis of particularly dangerous and dangerous infectious diseases is more extensive.
Table 2. Comparative characteristics of UIHE-1 kit and other methods of rapid pathogen indication [9]
Note: PCR — polymerase chain reaction; IHR — indirect haemoagglutination reaction; ELISA — enzyme linked immunosorbent assay.