ORIGINAL RESEARCH

Immunochromatography-based portable equipment for indication of pathogenic microorganisms and toxins

About authors

Federal State Unitary Enterprise "State Scientific Research Institute of Biological Engineering" FMBA of Russia (FSUE “SSRIBE”), Moscow, Russia

Corresponding author: Sergey P. Yarkov
Volokolamskoye, 75, str. 1, 125424, Moscow, Russia; ur.lod@losaid

About paper

Funding: the studies were carried out under government contracts with the Russian Federal Medical and Biological Agency (No. 42.128.11.6, No. 42.133.12.6) and the Russian Ministry of Health (No. K-27-FTP/82-1).

Contributing authors: Yarkov SP ― idea, development planning, analysis of findings, participation in tests, manuscript preparation; Shilenko IV ― creation of multi-analysis lateral flow immunoassays, implementation of tests of EkB sets, analysis of obtained results; Tretyakov SI ― creation of lateral flow immunoassays, models and kits, tests, analysis of research results; Ishkov YN ― study management, manuscript editing; Stiazhkin KK ― management, manuscript editing.

Received: 2022-11-26 Accepted: 2022-12-18 Published online: 2022-12-29
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This review looks at analytical capabilities and composition of portable equipment based on lateral flow immunoassay for rapid indication of human pathogenic bacteria, viruses and toxins which was developed by the State Research Institute of Biological Instrumentation under the auspices of the Federal Medical and Biological Agency of Russia. The review presents technical characteristics and composition of portable test kits UIHE-1 designed for taking monoanalytical and multi-analytical lateral flow immunoassay on pathogenic microorganisms and toxins in washes from environmental objects surfaces and in culture media; it also describes kits EkB and EkB-01 for analysis of biological aerosol samplers contents.  Information is given on the analytical properties of luminescence lateral flow immunoassay kit ULI-1, an on the experimental prototype of fluorimeter-reflectometer "Zondazh".  The technical characteristics of indication kits were compared with those of foreign origin, areas for improvement of portable equipment based on lateral flow immunoassay were indicated.

Keywords: identification, pathogenic bacteria, viruses, toxins, immunochromatography, sets and kits

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