ORIGINAL RESEARCH

Regulatory T cells and T helper 17 cells expressing CD39 and CD73 ectonucleotidase in children with severe injury

Zakirov RSh1,2, Kuptsova DG1, Freidlin EV1, Semikina EL1, Petrichuk SV1, Karaseva OV1,2
About authors

1 National Medical Research Center for Children's Health, Moscow, Russia

2 Institute of Urgent Children Surgery and Traumatology, Moscow, Russia

Correspondence should be addressed: Rustam Shakirovich Zakirov
Lomonosovsky prospect, 2/1, Moscow, 119296, Russia; ur.dzcn@hsr.vorikaz

About paper

Funding: the study was supported under the State Assignment by the Ministry of Health of Russia, #AAAA–A19–119021190051–6, #122040800163–9

Acknowledgments: the authors express their gratitude to all patients who participated in the study, as well as to colleagues from the department of concomitant injury, anesthesiology and resuscitation of the Research Institute of Emergency Pediatric Surgery and Traumatology of the Moscow Department of Health for their cooperation.

Author contribution: Zakirov RSh, Karaseva OV, Petrichuk SV — study planning, analysis of literature, collection of experimental data, analysis and interpretation of the results, manuscript authoring and editing; Semikina EL — study planning; Kuptsova DG, Freidlin EV — collection of experimental data.

Compliance with the ethical standards: the study was approved by the Ethics Committee of the Institute of Urgent Children Surgery and Traumatology of the Department of Health of Moscow (Minutes #2 of May 26, 2020). Parents of all participants of the study have signed the informed consent form in accordance with the principles of the Declaration of Helsinki.

Received: 2022-11-21 Accepted: 2022-12-11 Published online: 2022-12-24
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  1. Timofeev VV, Bondarenko AV. Epidemiological aspects of polytrauma in children in major city. Polytrauma. 2012; (4): 5–8. Russian.
  2. Bondarenko AV The organization of specialized care for polytrauma in a large city. N.N. Priorov Journal of Traumatology and Orthopedics. 2005; (4): 81–84. Russian.
  3. Cauwels A, Rogge E, Vandendriessche B, Shiva S, Brouckaert P. Extracellular ATP drives systemic inflammation, tissue damage and mortality. Cell Death Dis. 2014; 5 (3): e1102.
  4. Moro N, Ghavim SS, Sutton RL. Massive efflux of adenosine triphosphate into the extracellular space immediately after experimental traumatic brain injury. Exp Ther Med. 2021; 21 (6): 575.
  5. Faroqi AH, Lim MJ, Kee EC, Lee JH, Burgess JD, Chen R, Di Virgilio F, Delenclos M, McLean PJ. In Vivo Detection of Extracellular Adenosine Triphosphate in a Mouse Model of Traumatic Brain Injury. J Neurotrauma. 2021; 38 (5): 655–64.
  6. Golovkin AS, Serebryakova MK, Zhiduleva EV, Murtazalieva PM, Titov VA, Irtuga OB, Moiseeva OM, Krobinec II, Kudryavtsev IV. Purinergic signaling receptors expression on peripheral T-lymphocites of healthy donors. Translational Medicine. 2017; 4 (5): 46–60. Russian.
  7. Takenaka MC, Robson S, Quintana FJ. Regulation of the T Cell Response by CD39. Trends Immunol. 2016; 37 (7): 427–39.
  8. Timperi E, Barnaba V. CD39 Regulation and Functions in T Cells. Int J Mol Sci. 2021; 22 (15): 8068.
  9. Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+Treg cells: Hydrolysis of extracellular ATP and immune suppression. Blood. 2007; 110 (4): 1225–32.
  10. Zhang C, Li L, Feng K, Fan D, Xue W, Lu J: ‘Repair’ Treg Cells in Tissue Injury. Cell Physiol Biochem. 2017; 43: 2155–69.
  11. Sturm R, Xanthopoulos L, Heftrig D, Oppermann E, Vrdoljak T, Dunay IR, et al. Regulatory T Cells Modulate CD4 Proliferation after Severe Trauma via IL10. J Clin Med. 2020; 9 (4): 1052.
  12. Zhang Y, Li XF, Wu W, Chen Y. Dynamic changes of circulating T-helper cell subsets following severe thoracic trauma. Int J Clin Exp Med. 2015; 8 (11): 21106–13.
  13. Hein F, Massin F, Cravoisy-Popovic A, Barraud D, Levy B, Bollaert PE, et al. The relationship between CD4+CD25+CD127- regulatory T cells and inflammatory response and outcome during shock states. Crit Care. 2010; 14 (1): R19.
  14. Han L, Sugiyama H, Zhang Q, Yan K, Fang X, McCormick TS, et al. Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4+ CD25high Foxp3+ regulatory T-cells in psoriasis. Australas J Dermatol. 2018; 59 (1): e31–e38.
  15. Holmes JF, Palchak MJ, MacFarlane T, Kuppermann N. Performance of the pediatric Glasgow coma scale in children with blunt head trauma. Acad Emerg Med. 2005; 12: 814.
  16. Karaseva O, Roshal L. Pediatric Trauma in Earthquakes: General Principles of Care in Pediatric Trauma During Earthquakes. In: Wolfson N, Lerner A, Roshal L, eds. Orthopedics in Disasters: Orthopedic Injuries in Natural Disasters and Mass Casualty Events. Berlin Heidelberg: Springer, 2016.
  17. Zhou X, Yao J, Lin J, Liu J, Dong L, Duan M. Th17/Regulatory T-Cell Imbalance and Acute Kidney Injury in Patients with Sepsis. J Clin Med. 2022; 11 (14): 4027.
  18. Radygina TV, Kuptsova DG, Petrichuk SV, et al. Expression of CD39 and CD73 ectonucleotidases in CD4+ lymphocyte populations in healthy children. Russian Journal of Immunology. 2022; 25 (3): 283– 290. Russian.
  19. Vaara ST, Hollmén M, Korhonen AM, Maksimow M, Ala-Kokko T, Salmi M, et al. FINNAKI Study Group. Soluble CD73 in Critically Ill Septic Patients — Data from the Prospective FINNAKI Study. PLoS One. 2016; 11 (10): e0164420.
  20. Zhai X, Chen K, Yang H, Li B, Zhou T, Wang H, et al. Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury. J Nanobiotechnology. 2021; 19 (1): 274.