2023 / 02

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DOI: 10.47183/mes.2023.011

METHOD

Assessing the possibility of interactions of various metals with alpha-2-macroglobulin and other human blood proteins in vitro

Zorina VN, Evdokimova EA, Rejniuk VL
About authors

Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency, Saint-Petersburg, Russia

Correspondence should be addressed: Veronika N. Zorina
Bekhtereva, 1, Saint-Petersburg, 192019, Russia; ur.xednay@nummilin

About paper

Acknowledgements: the authors would like to thank Professor N.A. Zorin for developing the variants of the crossed immunoelectrophoresis with in situ adsorption in the second dimension and the method for extraction of the highly purified α2M preparation, as well as for providing the antisera samples.

Author contribution: Zorina VN — planning and conducting the study, literature review, manuscript writing; Evdokimova EA — compliance check, compilation of reference list; Rejniuk VL — manuscript editing.

Compliance with ethical standards: the study was carried out in accordance with the principles stated in the Declaration of Helsinki of the World Medical Association.

Received: 2023-03-16 Accepted: 2023-04-07 Published online: 2023-05-18
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Fig. 1. Interaction of immobilized metal ions with serum proteins. The reduced height and area of the peak are indicative of interaction between protein and metal contained in intermediate gel. The layout of metals in the samples is in table
Fig. 2. Interaction of immobilized metal ions with alpha-2-macroglobulin from blood serum. The reduced height and area of the peak are indicative of interaction between protein and metal contained in intermediate gel. The layout of metals in the samples is in table
Table 1. List of substances used for immobilization of metals
Table 2. Binding affinity of human serum proteins to the studied metal ions contained in intermediate gel during immunoelectrophoresis