METHOD
Assessing the possibility of interactions of various metals with alpha-2-macroglobulin and other human blood proteins in vitro
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency, Saint-Petersburg, Russia
Correspondence should be addressed: Veronika N. Zorina
Bekhtereva, 1, Saint-Petersburg, 192019, Russia; ur.xednay@nummilin
Acknowledgements: the authors would like to thank Professor N.A. Zorin for developing the variants of the crossed immunoelectrophoresis with in situ adsorption in the second dimension and the method for extraction of the highly purified α2M preparation, as well as for providing the antisera samples.
Author contribution: Zorina VN — planning and conducting the study, literature review, manuscript writing; Evdokimova EA — compliance check, compilation of reference list; Rejniuk VL — manuscript editing.
Compliance with ethical standards: the study was carried out in accordance with the principles stated in the Declaration of Helsinki of the World Medical Association.
- Wang F, Chmil C, Pierce F, Ganapathy K, Gump B, MacKenzie J, Mechref Y, Bendinskas K. Immobilized metal affinity chromatography and human serum proteomics. Journal of Chromatography B. 2013; 934: 26–33.
- Witkowska D, Rowińska-Żyrek M, Witkowska D, RowińskaŻyrek M. Biophysical approaches for the study of metal-protein interactions. Journal of Inorganic Biochemistry. 2019; 199: 110783.
- Lozhkin AP, Biktagirov TB, Abdulyanov VA, Gorshkov OV, Timonina EV, Mamin GV, i dr. Marganec v aterogeneze: obnaruzhenie, proisxozhdenie i rol'. Biomedicinskaya ximiya. 2012; 58 (3): 291– 99. Russian.
- Aschner M, Costa LG. Neurotoxicity of Metals. Springer International Publishing AG, 2017; 383 p.
- Ruseckaya NYu, Borodulin VB. Biologicheskaya aktivnost' selenorganicheskix soedinenij pri intoksikacii solyami tyazhelyx metallov. Biomedicinskaya ximiya. 2015; 61 (4): 449–61. Russian.
- Zorina VN, Zorina RM, Zorin NA. Osobennosti vzaimodejstviya belkov semejstva makroglobulinov mezhdu soboj i s receptorami ehndocitoza (vozmozhnyj mexanizm transmembrannogo perenosa). Biomedicinskaya ximiya. 2011; 57 (1): 106–13. Russian.
- Barashkov GK, Zajceva LI, Kondaxchan MA, Konstantinova EA, Raspredelenie ximicheskix ehlementov v cel'noj krovi i plazme. Biomedicinskaya ximiya. 2003; 49 (3): 297–302. Russian.
- Zorin NA, Zorina RM, Zorina VN. Poluchenie preparatov α-makroglobulina s zadannymi svojstvami. Gematologiya i transfuziologiya. 2000; 45 (5): 20–21. Russian.
- Emmet M, Crowle AJ. Crossed immunoelectrophoresis: qualitative and quantitative considerations. J Immunol Meth. 1982; 50: R65–R83.
- Manley SA, Byrns S, Lyon AW, Brown P, Gailer J. Simultaneous Cu-, Fe-, and Zn- specific detection of metalloproteins contained in rabbit plasma by size-exclusion chromatography–inductively coupled plasma atomic emission spectroscopy. Biol Inorg Chem. 2009; 14: 61–74.
- Cheknyov SB. Belki γ-globulinovoj frakcii, xelatiruyushhie kationy metallov, v fiziologicheskoj immunoregulyacii. Oppozitnye ehffekty medi i cinka. Immunologiya. 2021; 42 (3): 293–300. Russian.
- Genchi G, Sinicropi M, Lauria G, Carocci A, Catalano A. The Effects of Cadmium Toxicity. Int J Environ Res Public Health. 2020; 17 (11): 3782.
- Ynalvez R, Gutierrez J, Gonzalez-Cantu H. Mini-review: toxicity of mercury as a consequence of enzyme alteration. Biometals. 2016; 29 (5): 781–8.
- Koshkina VS. Kotlyar NN, Kotelnikova LV, Dolgushina NA. Kliniko-toksikologicheskaya xarakteristika svinca i ego soedinenij. Medicinskie novosti. 2013; 1: 20–25. Russian.
- Mocchegiani E, Costarelli L, Giacconi R, Cipriano C, Muti E, Malavolta M. Zinc-binding proteins (metallothionein and a-2 macroglobulin) and immunosenescence. Experimental Gerontology. 2006; 41: 1094–7.
- Assi MA, Hezmee MN, Haron AW, Sabri MY, Rajion MA. The detrimental effects of lead on human and animal health. Vet World. 2016; 9 (6): 660–71.
- Mazunina DL. Negativnye ehffekty marganca pri xronicheskom postuplenii v organizm s pit'evoj vodoj. Ehkologiya cheloveka. 2015; 3: 25–31. Russian.
- Heeb MJ, Gruber A, Griffin JH. Identification of Divalent Metal Iondependent Inhibition of Activated Protein Cbycr2-Macroglobulin and cr2-Antiplasminin Blood and Comparisons to Inhibition of Factor Xa, Thrombin, and Plasmin. The Jounal of Biological chemistry. 1991; 266 (26): 17606–12.