2023 / 02

Next Paper
DOI: 10.47183/mes.2023.017

ORIGINAL RESEARCH

Therapeutic efficacy of N-cholinolitic drug N,N-Diethyl-5,5-diphenyl-2-pentynylamine in models of non-cardiogenic pulmonary edema

Melikhova MV, Krasnov KA, Rozhko MA, Lapina NV, Krasnova AA
About authors

Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency, Saint-Petersburg, Russia

Correspondence should be addressed: Konstantin A. Krasnov
Bekhtereva, 1/2, k. 54, Saint-Petersburg, 199106, Russia; ur.liam@xot_vonsark

About paper

Funding: state contract № 2124388100122000000000000/64.020.21.9 of 2 November 2021, “Developing New Antidote Medications for Treatment of Toxic Pulmonary Edema” (code: “Tuman”)

Author contribution: Melikhova MV — assessment of the animals’ physiological state, data processing and interpretation; Krasnov КА — modeling of toxic pulmonary edema in animals, prepress manuscript preparation; Rozhko МА — drug administration through inhalation and monitoring of external respiration parameters in animals; Lapina NV — general management; Krasnova АА — lung gravimetry in animals.

Compliance with ethical standards: all the procedures involving model animals were performed in accordance with the principles of Good Laboratory Practice and the Directive 2010/63/EU of the European Parliament and (2010) on the protection of animals used for scientific purposes.

Received: 2023-03-31 Accepted: 2023-05-31 Published online: 2023-06-14
|
Fig. Chemical structure of DDPA
Table 1. The effects of DDPA on the thiourea-induced NCPE manifestations (M ± m; n = 20)
Note: * — significant difference from control (p < 0.05)
Table 2. The effects of DDPA on the survival rate of rats with NCPE induced by NO2 inhalation (M ± m; n = 10)
Note: * — significant difference from control (p < 0.05)
Table 3. The effects of DDPA on the external respiration parameters in rats with NCPE induced by NO2 inhalation (LC50/20min = 210 mg/m3) (M ± m; n = 12)
Note: * — significance of differences between the baseline values and the values obtained within 2 and 5 h (p < 0.05)
Table 4. The effects of DDPA on gravimetric pulmonary coefficients of rats with NCPE induced by NO2 inhalation (LC50/20min = 210 mg/m3) (M ± m; n = 12)
Note: * — significance of differences between intact and experimental groups (р < 0.05)