ISSN Print 2713-2757
ISSN Online 2713-2765
Extreme Medicine
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Frequency of inversions in the T-lymphocyte chromosomes of exposed residents of the Southern Urals
About authors
Urals Research Center for Radiation Medicine of the Federal Medical Biological Agency, Chelyabinsk, Russia
Correspondence should be addressed: Yana V. Krivoshchapova
Vorovsky, 68А, Chelyabinsk, 454141, Russia; ur.liam@oh_anay
About paper
Funding: the study was supported in part by the Russian Foundation for Basic Research (RFBR) grant together with the Government of the Chelyabinsk Region (Contract № 20-44-740007\20 of 28.01.2021), as well as by the Federal Target Program of FMBA of Russia “Modernization of Hightech Methods Aimed at Identifying Medical Consequences of Radiation Exposure in Personnel of the Mayak PA and the Population of the Ural Region” (code “Medical Consequences-21”).
Acknowledgments: the authors would like to express their gratitude to Savkova N.F., senior laboratory assistant, for technical and laboratory support.
Author contribution: Vozilova AV — study concept, research priority setting, literature review, manuscript writing; Krivoshchapova YaV — developing criteria for analysis, staining and slide assessment, statistics, manuscript writing.
Compliance with the ethical standards: the study was approved by the Ethics Committee of the Urals Research Center for Radiation Medicine (protocol No. 7 dated 20 October 2023); individuals, who were through cytogenetic testing, submitted the informed consent to blood sampling and further assessment.
Received: 2023-08-08
Accepted: 2023-10-15
Published online: 2023-11-21
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It is well-known that ionizing radiation is among factors increasing the rate of chromosomal rearrangements. The inversion rate was poorly understood due to difficulty of inversion identification by the conventional differential staining method. A comprehensive study of chromatin and its complex rearrangements has become possible with the use of the high-tech molecular genetic method, fluorescence in situ hybridization (FISH). The study was aimed to assess frequency of inversions involving the chromosome telomeric regions in 36 residents of the South Urals, almost all of them were affected by combined chronic exposure. The calculated individualized cumulative external and internal doses were 0.0001–4.7 Gy. Inversions were identified by fluorescence staining of the chromosome telomeric region. It was found that chromatid inversions were more abundant than chromosomal variants (9 : 0.3 per 100 cells (p < 0.001). No relationship between the studied parameters and the absorbed dose, sex and age at the time of the examination was revealed.
Keywords: ionizing radiation, FISH, Techa River, chromosomal aberrations, inversions, telomeric regions of chromosomes