ISSN Print 2713-2757
ISSN Online 2713-2765
Extreme Medicine
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Effect of cystamine on gastric propulsive function and gas exchange in the rat model of radiation-induced myeloablation
About authors
1 Golikov Research Clinical Center of Toxicology of the Federal Medical Biological Agency, Saint-Petersburg, Russia
2 State Scientific Research Test Institute of the Military Medicine of Defense Ministry of the Russian Federation, Saint-Petersburg, Russia
Correspondence should be addressed: Timur V. Schäfer
Lesoparkovaya, 4, Saint-Petersburg, 195043, Russia; ur.xednay@refahcs
About paper
Author contribution: Vakunenkova OA — experimental procedure; Ivnitsky JuJu — rationale, developing the experimental model, data interpretation and discussion; Danilova OA — tissue biochemistry studies; Schäfer TV — experimental procedure, data processing and visualization, developing the experimental model; Rejniuk VL — methodological guidance of gas exchange assessment. All authors contributed to discussion, manuscript writing and editing.
Compliance with the ethical standards: the study was carried out in accordance with the principles of bioethics, approved by the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes.
Received: 2023-09-29
Accepted: 2023-11-20
Published online: 2023-12-07
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Fig. 1. Abdominal organs of rats deprived of food 48 h before laparotomy. А. Intact. B. 72 h after single total body X-ray exposure at a dose of 9.64 Gy. Arrows: 1 — stomach; 2 — caecum
Fig. 2. Mass indexes of gastric chyme (А), caecal chyme (B) and their ratio (C) in rats 72 h after the single total body X-ray exposure at a dose of 9.64 Gy, M ± m, n = 8, depending on the duration of access to food since the time of irradiation. Control — non-exposed animals. Values of the group of non-exposed rats which had the unlimited access to food are at the zero mark of horizontal axis. * — significant difference from control, p < 0.05
Fig. 3. Mass indexes of gastric chyme, caecal chyme and the spleen (А), urinary indican excretion (B), cholinesterase (C) and alkaline phosphatase (D) activity in the intestinal tissues of rats 72 h after the single total body X-ray exposure to the dose of 9.64 Gy, M ± m, n = 8, depending on the duration of access to food since the time of irradiation. “Intact” — non-exposed rats which obtained no medication. “Irradiation” — rats exposed without administration of radioprotector. ”Cystamine” — intragastric administration of cystamine dihydrochloride in a dose of 120 mg/kg 30 min before the beginning of irradiation. All animals were deprived of food 24 h after irradiation. Significant difference, p < 0.05: * — from intact group; † — from “Irradiation” group
Fig. 4. The whole body oxygen consumption (А), respiratory rate (B) and oxygen consumption per respiratory cycle (C) in rats 72 after the single total body X-ray exposure at a dose of 9.64 Gy, M ± m, n = 8. QO2 — the whole body oxygen consumption; RR — respiratory rate. 100% is the parameter values determined at 4 h before irradiation. * — significant difference from baseline, p < 0.05; † — significant difference from intact group, p < 0.05
