ISSN Print 2713-2757
ISSN Online 2713-2765
Extreme Medicine
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Combined matrices and tissue-engineered constructs made of biopolymers in reconstructive surgery of ENT organs
About authors
1 National Medical Research Center for Otorhinolaryngology of the Federal Medical Biological Agency, Moscow, Russia
2 National University of Science and Technology MISIS, Moscow, Russia
3 TrioNova LLC, Moscow, Russia
4 Imtek LLC, Moscow, Russia
5 Priorov National Medical Research Center of Traumatology and Orthopaedics, Moscow, Russia
6 3D Bioprinting Solutions, Moscow, Russia
Correspondence should be addressed: Sergey S. Reshulsky
Volokolamskoe shosse, 30/2, Moscow, 123182, Russia; ur.liam@50SSR
About paper
Author contributions: Daikhes NA — concept, planning the experiment, management, manuscript editing; Diab KhM — manuscript writing, data provosion; Nazaryan DN — surgical stage of the experiment, manuscript editing; Vinogradov VV, Reshulsky SS — manuscript writing, data acquisition; Machalov AS — planning the experiment, data acquisition, manuscript editing; Karshieva SSh — creating the endoprosthesis, cell culture maintenance; Zhirnov SV — creating the endoprosthesis, printing the substrate; Osidak EO — creating the endoprosthesis, developing the hydrogel; Kovalev AV — histological assessment; Hesuani YuD — creating the resulting tissue-engineered construct.
Соблюдение этических стандартов: все манипуляции с животными были проведены в соответствии c едиными этическими нормами Базельской декларации.
Received: 2023-12-20
Accepted: 2024-02-14
Published online: 2024-03-25
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Fig. 1. Flowchart showing phases of the experiment
Fig. 2. Surgical wound after implantation of bioengineered ear, “Cylinder 1” and “Cylinder 2”. а — site of the bioengineered ear implantation in the region of the right temporal fascia; b — site of the “Cylinder 1” (collagen + autologous chondrocytes) implantation in the region of the left temporal fascia; c — site of the “Cylinder 12” (collagen) implantation in the region of frontalis muscle
Fig. 3. Bioengineered ear (А), “Cylinder 1” (collagen + autologous chondrocytes) (B) and “Cylinder 2” (collagen) (C) after harvesting, 3 months after implantation
Fig. 4. Histological specimen of bioengineered ear, hematoxylin and eosin stain (А); Mallory stain (B); immunohistochemistry specimen (C)
Fig. 5. Histological specimen of “Cylinder 1” based on type 1 collagen with autologous chondrocytes: hematoxylin and eosin stain (А); Mallory stain (B); immunohistochemistry specimen (C)
Fig. 6. Histological specimen of “Cylinder 2”: hematoxylin and eosin stain (А); Mallory stain (B); immunohistochemistry specimen (C)
Table 1. Comparative characteristics of histological specimens obtained from both animals
Note: +++– — parameter is strongly expressed; ++– – — parameter is moderately expressed; +– – — parameter is weakly expressed; – – – — parameter is not expressed.
