ISSN Print 2713-2757
ISSN Online 2713-2765
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Method to assess the effects of bioactive compounds solutions on blood clotting
About authors
1 Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical Biological Agency, Moscow, Russia
2 Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow Region, Russia
Correspondence should be addressed: Valentin A. Manuvera
Malaya Pirogovskaya, 1A, Moscow, 119435, Russia; ur.xednay@arevunamv
About paper
Funding: the study was supported by the Russian Science Foundation grant No. 23-25-00006, https://rscf.ru/project/23-25-00006/.
Author contribution: Manuvera VA — concept, experiments, manuscript writing; Brovina KA — experiments, manuscript editing; Bobrovsky PA — data analysis, visualization, manuscript editing; Grafskaia EN — experiments, manuscript editing; Kharlampieva DD — experiments, manuscript editing; Lazarev VN — research team management, manuscript editing.
Received: 2024-03-27
Accepted: 2024-06-15
Published online: 2024-06-28
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Fig. 1. Results for aPTT, PT, and TT measurements for the solutions of three anticoagulants: cysteine-rich anticoagulant (CRA) derived from the medicinal leech, hirudin (Hir), and heparin (Hep). Concentrations of CRA and Hir are measured in µM of protein in the test solution; the total amount of IU per reaction is provided for Hep. The mean clot formation time for the control sample (10 mМ TrisCl, pH = 7.5) is shown as the dashed line. The data are provided as the mean values of clot formation time and standard deviations. Four independent measurements per point were performed
Table. Effects of various solutions on the aPTT, TT, and PT determination
Note: mean clot formation time in seconds ± standard deviation is provided; 1 — phosphate buffer solution (20 mМ sodium phosphate, 8 g/L sodium chloride, pH = 7.4); 2 — no clot was formed, no value is available.
