Extreme Medicine

ISSN Print 2713-2757 ISSN Online 2713-2765

Extreme Medicine

SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA

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Petrash EA, Nikishina VB, Razuvaeva TN, Sokolyskaya MV, Kuznetsova AA, Zapesotskaya IV

The relevance of this study is due to the need to answer the question of how the factors of digital medium affect the development of mental functions in the younder generation. The study was aimed to assess the effects of the social media interference factor on memory (auditory-speech and visual-figurative) consolidation in adolescents. The sample was 130 adolescents aged 11–17. The groups were formed based on the age stages (11–12, 13–14, 15–17 years). The study involved the use of the method for the diagnosis of the short-term auditory-speech and visual-figurative memory span. Viewing video content and reading the fragment of the fiction book were used as interference. A significant decrease in the short-term auditory-speech and visual-figurative memory span was observed in adolescents. The short-term memory span is reduced in the context of social media interference (prolonged continuous viewing the heterogenous visual-acoustic and visualspeech content). The high risk of the long-term memory loss due to the impact of the social media interference factor on memory consolidation in adolescents is empirically proven. The more the duration of the maximum continuous video stream and the total time the adolescents spend on Internet (including social media), the larger is the loss of information.

Received: 2022-10-13

Published online: 2022-12-16

DOI: 10.47183/mes.2022.039

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VIEWS 1488

Gorodnichev RB, Kornienko MA, Bespiatykh DA, Malakhova MV, Veselovsky VA, Goloshchapov OV, Chukhlovin AB, Bespyatykh JA, Shitikov EA

Bacterial infections caused by antibiotic resistant strains of Klebsiella pneumoniae are among the most dangerous threats for the world's public healthcare. Treatment with bacteriophages and/or their derivatives could become one of the alternative methods for therapy of infections caused by K. pneumoniae. The study was aimed to isolate from the environment and characterize the capsule-specific K. pneumoniae bacteriophages that are useful for therapy and possess the polysaccharide depolymerase genes. Bacteriophages were isolated from the river water samples by enrichment method. The host range of bacteriophages were assessed using the collection of 180 K. pneumoniae clinical strains. Bacteriophage whole genome sequencing was performed on the MiSeq platform (Illumina). Four new bacteriophages from different taxonomic groups were isolated and characterized during the study: vB_KpnM_NDO71 (Vequintavirinae family), vB_KpnS_MAG26fr (Casjensviridae family), vB_KpnS_MDA2066 (Ackermannviridae family), and vB_KpnS_PMM-G3 (Drexlerviridae family). Bacteriophages vB_KpnM_NDO71, vB_KpnS_MAG26fr, and vB_KpnS_PMM-G3 had a narrow lytic spectrum and lysed all strains with the capsular type of the host: KL45, KL19 or KL28, respectively. Bacteriophage vB_KpnS_MDA2066 showed lytic activity against strains with two different capsular types: KL19 and KL107. Bacteriophages were strictly virulent and contained no integrase genes, potentially dangerous toxin genes or antibiotic resistance determinants. This allows them to be used in therapeutic practice. Receptor-binding proteins represented by polysaccharide depolymerases were predicted for each bacteriophage.

Received: 2022-11-05

Published online: 2022-12-03

DOI: 10.47183/mes.2022.038

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VIEWS 1456

Eremeev AV, Pikina AS, Ruchko ES, Sidorov VS, Ragozin AO

One of the approaches to cartilage tissue restoration problem relies on cellular technologies that use iPSCs, induced pluripotency stem cells that are an unlimited source of cellular material for tissue engineering with significant differentiation potential. However, there are no standardized protocols for chondrogenic differentiation of iPSCs. This study aimed to make cartilage tissue samples using 3D spheroid cultures and following four chondrogenic differentiation protocols, then compare characteristics of the cartilage samples made under different protocols and isolate the most effective way of differentiation. The iPSCs were differentiated chondrogenically, the four protocols were "long", "short", "combined" and with conditioned medium from a primary culture of autologous chondrocytes; the combinations of TGFβ1, BMP2, Chir 99021, and PK factors varied. Microwell plates were used to make spheroids. Immunocytochemical staining, real-time PCR and histological staining enabled assessment of the synthesis and expression profiles. High rates of synthesis and expression of chondrogenic markers Sox9, aggrecan, type II collagen were observed in spheroids experimented with under the "long", "combined" protocols and the conditioned medium protocol. The "combined" differentiation protocol made chondrogenesis most effective, and conditioned medium was highly efficient in inducing and supporting chondrogenic differentiation.

Received: 2022-10-12

Published online: 2022-11-23

DOI: 10.47183/mes.2022.037

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VIEWS 1981

Patrakeeva VP, Schtaborov VA

The rapid switch on of the transient short-term responses involved in adjustment of homeostasis plays a key role in human adaptation to low temperatures that is essential for adjustment to low-temperature environment. The network of signaling pathways together with metabolic regulators provide sufficient plasticity of the cells of immune system, the normal function of which is extremely important for successful human adaptation. Sufficient energy supply to immunocompetent cells makes it possible to form an adequate immune response to any negative factor and to ensure adaptive functional rearrangements. The study was aimed to assess the variants of the immunocompetent cell metabolic pathways involved in acquiring individual cold sensitivity. A total of 180 people aged 25–55 (130 females, 50 males) were assessed before and after the short-term whole body cooling. Enzyme immunoassay was used to define the levels of IL10, IL6, TNFα, irisin, transferrin, sTfR, HIF-1α, Sirt3 in peripheral blood and cell lysate. The levels of glycogen (cytochemical methods) and ATP (luciferin-luciferase assay) in lymphocytes were defined. The decrease in peripheral blood lymphocyte levels after cooling was indicative of the formation of immediate adaptive response and activation of glycolysis amid less intense inflammatory response. The increase in the levels of circulating lymphocytes after the cold esposure was associated with activation of inflammatory responses. The lower ratio of HIF-1α/SIRT3 metabolic regulators was found in the surveyed volunteers who showed no changes in the levels of lymphocytes. This indicated predominance of mitochondrial activity in adaptation to low temperatures.

Received: 2022-10-14

Published online: 2022-11-17

DOI: 10.47183/mes.2022.036

Comments 0

VIEWS 1483

Saltykova MM, Antipina UI, Balakaeva AV

Mortality rate is one of the main indicators of how healthy a population is, and planning and implementing measures aimed at reducing morbidity and increasing life expectancy in the population is impossible without an adequate analysis and interpretation of mortality data. At the same time, as pointed out by many researchers, there are factors external to a human body being that can have a significant effect on the mortality rate in a population. This study aimed to assess the impact of one of these factors, the number of beds in hospitals (per 10,000 people) of cities with population exceeding 100,000 people. The analysis included data from Rosstat (Russian statistics service) on the population size, mortality, number of hospital beds, average monthly wages in 12 cities within the period from 2017 through 2019. Five cities from these 12 were selected as a more homogeneous subgroup in terms of socio-economic conditions. We found a positive correlation between mortality rate per 1000 inhabitants (R > 0.7; p < 0.009) and the number of hospital beds per 10,000 people in the sample of 12 cities. This correlation was higher (R ≥ 0.9; p < 0.037) in the more homogeneous subgroup. A factor that may condition this correlation may be that of deaths of people from other regions in hospitals of the cities in question, which are counted when estimating the mortality rate and have a significant effect on that estimation. The results of the study point to the need to differentiate between people registered in a city and those living there permanently when assessing mortality rate therein.

Received: 2022-09-21

Published online: 2022-11-07

DOI: 10.47183/mes.2022.035

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VIEWS 1305

Rabdano SO, Mukhin VE, Makarov VV, Rudakov GO, Ruzanova EA, Arakelov SA, Khaitov MR, Yudin SM, Kryuchko DS, Berzin IA, Evtushenko AE, Trukhin VP, Skvortsova VI

The second generation COVID-19 vaccines should produce the long-term protective immune response to the existing and novel strains of SARS-CoV-2. The Convacell® vaccine was designed to produce such immune response by using N protein as an antigen. N-protein is not susceptible to fast accumulation of mutations and is highly homologous to nucleocapsid proteins of other β-coronaviruses. The study was aimed to perform in vitro assessment of the Convacell® vaccine ability to produce immune response to the Wuhan, Delta, and Omicron strains. Mononuclear cells of vaccinated volunteers and survivors were subjected to N protein stimulation. After that specific activation of the cells was assessed by flow cytometry. The results showed that a sibstantial percentage of CD4 and CD8 cells produced IFNγ and IL2 in response to stimulation. No significant reduction of the response to strains Delta and Omicron compared to the Wuhan strain was revealed. The findings support the direction of the N protein based vaccine design towards creation of the universal vaccine.

Received: 2022-09-12

Published online: 2022-09-30

DOI: 10.47183/mes.2022.033

Comments 0

VIEWS 2129

Diab KhMA, Daykhes NA, Pashchinina OA, Shamkhalova AM, Panina OS

The review of papers, focused on studying various neoplasms, diagnosis, selection of surgical approach, complications, and recurrence rates of the petrous apex lesions, that have been published in 2008–2022, is provided. Effusion, mucocele, cholesterol granuloma, cholesteatoma are the most common benign lesions of the petrous apex. Such surgical approaches as translabyrinthine, transcochlear, endoscopic endonasal approach and the middle cranial fossa approach are most often used during treatment. The middle cranial fossa approach, infracochlear approach and endoscopic transnasal approach are recommended for patients with preserved hearing. In case of disseminated lesions, when the carotid artery and the jugular bulb should be additionally controlled, transcochlear and translabyrinthine surgical corridors could be used.

Received: 2022-08-19

Published online: 2022-09-29

DOI: 10.47183/mes.2022.032

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VIEWS 1402

Blinova EA, Kotikova AI, Akleyev AA, Akleyev AV

Currently, cancer is the major cause of mortality and disability among the working age population of the developed countries. Early diagnosis of tumors, that involves monitoring the health of people exposed to radiation, is one of the most pressing challenges faced by radiation medicine. The study was aimed to perform quantification and functional assessment of the system of neutrophil granulocytes, monocytes and natural killers (NK cells) in people who were diagnosed with tumors after chronic radiation exposure. Certain factors of innate immunity were assessed in 104 people, chronically exposed to low-dose radiation over a wide dose range. Of them 34 exposed individuals were later diagnosed with malignant tumors (MTs). We assessed the number of white blood cells, neutrophil granulocytes, eosinophils, basophils, monocytes and NK cells (CD16+/CD56+ lymphocytes) in peripheral blood, as well as phagocytic, lysosomal activity and intracellular oxygen-dependent metabolism of neutrophils and monocytes. Individuals, chronically exposed a few years before the development of MTs, showed a significant increase in the phagocytosis rate of monocytes (median 10.50 AU vs. 6 AU; p = 0.05) and lysosomal activity of neutrophils (median 482 AU vs. 435.5 AU; p = 0.03) compared to patients with no MTs. Assessment of the the dose–response relationship in exposed people, who subsequently developed cancer, revealed a significant increase in the phagocytosis rate of monocytes as a function of the accumulated dose to thymus and peripheral lymphoid organs (ρ = 0.45; p = 0.009), and the increase in phagocytic activity of neutrophils with the increase in the accumulated dose to red bone marrow (ρ = 0.44; p = 0.01).

Received: 2022-08-26

Published online: 2022-09-29

DOI: 10.47183/mes.2022.034

Comments 0

VIEWS 1544