ISSN Print 2713-2757
ISSN Online 2713-2765
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
BCL-2, CDKN1A and ATM gene methylation in chronically exposed individuals
About authors
1 Ural Research Center for Radiation Medicine, Chelyabinsk, Russia
2 Chelyabinsk State University, Chelyabinsk, Russia
Correspondence should be addressed: Eugenia A. Blinova
Vorovskogo, 68, korp. 1, Chelyabinsk, 454141; ur.mrcru@avonilb
About paper
Funding: the study was carried out within the framework of the State assignment of Russian Federal Medical Biological Agency “Human Cell-Mediated Immunity During Realization of Chronic Radiation Exposure Late Effects” (code 27.002.20.800).
Author contribution: Blinova EA — literature analysis, experimental procedure, data processing, manuscript writing; Nikiforov VS — literature analysis, experimental procedure, manuscript writing; Yanishevskaya MA — experimental procedure, manuscript editing; Akleyev AV — general management, manuscript writing.
Compliance with ethical standards: the study was approved by the Ethics Committee of Ural Research Center for Radiation Medicine of Russian Federal Medical Biological Agency (protocol № 2 dated July 20, 2021).The informed consent was submitted by all examined individuals.
Received: 2021-07-27
Accepted: 2021-08-29
Published online: 2021-09-21
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Table 1. Characteristics of studied groups
Table 2. Characteristics of olygonucleotides used for analysis
Note: Tm — melting temperature; Meth — methylated primer; Unmeth — unmethylated primer.
Table 3. Rate of CpG islands' methylation in BCL-2, CDKN1A and ATM gene promoters in peripheral blood cells of chronically exposed individuals
Note: MP — number of individuals with methylated promoter; UMP — number of individuals with unmethylated promoter; F — Fisher's exact test; р — significance level.
