2022 / 04

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DOI: 10.47183/mes.2022.038

ORIGINAL RESEARCH

Isolation and characterization of Klebsiella pneumoniae bacteriophages encoding polysaccharide depolymerases with rare capsule specificity

Gorodnichev RB1, Kornienko MA1, Bespiatykh DA1, Malakhova MV1, Veselovsky VA1, Goloshchapov OV2, Chukhlovin AB2,3, Bespyatykh JA1, Shitikov EA1
About authors

1 Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia

2 Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia

3 Pediatric Research and Clinical Center of Infectious Diseases of the Federal Medical Biological Agency, Saint Petersburg, Russia

Correspondence should be addressed: Roman B. Gorodnichev
Malaya Pirogovskaya, 1а, Moscow, 119435, Russia; moc.liamg@b.r.vehcindorog

About paper

Funding: the study was supported by the funds of the State Assignment "Development of the Scheme for Complex Therapy of Infectious Diseases Caused by Antibiotic Resistant Pathogens Involving the Use of Bacteriophages or Their Derivatives in Combination with Antibacterials” (code: Bacteriophage-2). Typing of Klebsiella pneumoniae strains was supported by the Russian Science Foundation (project No. 22-15-00149, https://rscf.ru/project/22-15-00149/).

Author contribution: Gorodnichev RB, Kornienko MA — study plan, data acquisition and processing, manuscript writing; Bespiatykh DA — data processing; Malakhova MV — data acquisition; Veselovsky VA, Goloshchapov OV, Chukhlovin AB, Bespyatykh JA — data acquisition and processing, Shitikov EA — study plan, data processing, manuscript writing.

Compliance with ethical standards: experimental work was carried out in compliance with the guidelines SP 1.3.2322-08 "Safety of Working With Microorganisms of III—IV Groups of Pathogenicity (Danger) and Causative Agents of Parasitic Diseases"; guidelines SP 1.3.2518-09 “Additions and Amendments № 1 to the guidelines SP 1.3.2322-08 "Safety of Working With Microorganisms of III—IV Groups of Pathogenicity (Danger) and Causative Agents of Parasitic Diseases"; guidelines "Sanitary and Epidemiologic Requirements for the Handling of Medical Waste" (SanPiN 2.1.7.2790-10 SanPiN 3.3686-21, SanPiN 2.1.3684-21); Federal Clinical Guidelines "Rational Use of Bacteriophages in Clinical and Epidemiological Practice".

Received: 2022-11-05 Accepted: 2022-12-01 Published online: 2022-12-03
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