2023 / 01

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DOI: 10.47183/mes.2023.006

ORIGINAL RESEARCH

Assessment of individual hematopoietic stem cell response to gamma exposure using humanized mice

Atamanyuk NI1, Pryakhin EA1, Styazhkina EV1,2, Obvintseva NA1, Tryapitsyna GA1,2, Peretykin AA1, Andreev SS1, Aldibekova AE1, Akleyev AV1,2
About authors

1 Urals Research Center for Radiation Medicine of the Federal Medical Biological Agency, Chelyabinsk, Russia

2 Chelyabinsk State University, Chelyabinsk, Russia

Correspondence should be addressed: Natalia I. Atamanyuk
Vorovskogo, 68А, Chelyabinsk, 454141, Russia; ur.liam@arhlup_ativ

About paper

Funding: the study was performed as part of the State Assignment of FMBA of Russia.

Acknowledgments: the authors would like to thank A.V. Sherstobitov (Regional Perinatal Center, Chelyabinsk) for his help in organizing and cord blood sampling.

Author contribution: Atamanyuk NI — planning and conducting experiments, manuscript writing; Pryakhin EA — study planning and management, manuscript writing; Styazhkina EV — HSC isolation, data analysis; Obvintseva NA — measurement, flow cytometry; Tryapitsyna GA — data analysis; Peretykin AA — animal exposure, dosimetry studies; Andreev SS — animal handling, measurement; Aldibekova AE — animal handling; Akleyev AV — general management.

Compliance with the ethical standards: the study was approved by the Ethics Committee of the Urals Research Center for Radiation Medicine (protocol № 2 of 27 June 2022). Cord blood sampling was performed after obtaining the informed consent from donors at the Chelyabinsk Regional Perinatal Center. Peripheral blood samples were provided by the Blood Transfusion Station of FMBA of Russia (Chelyabinsk) in accordance with the Decree of the Government of the Russian Federation № 331 of 12 April 2013. Animals were handled in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (1986, Strasbourg), Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes.

Received: 2023-02-22 Accepted: 2023-03-09 Published online: 2023-03-11
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Fig. 1. Survival rate of the CD45lowCD34+ human cells in the model mice humanized with the cord blood HSCs as a function of the acute external gamma exposure dose. А. HSC survival rate three days after exposure. B. HSC number relative to the non-exposed control 14 days after exposure
Fig. 2. Relationship between the C14/3 coefficient and the dose for CD45lowCD34+ human cells in the model mice humanized with the cord blood HSCs
Fig. 3. C14/3 coefficient for the CD45lowCD34+ human cells in the model mice humanized with the cord blood HSCs isolated from three donors. Responses of human hematopoietic cells in humanized mice to radiation exposure in the groups administered (induced radioresistance) and not administered cysteamine. * — significant differences between groups, р < 0.05
Fig. 4. C14/3 coefficient for hematopoietic CD117+ cells of the lines of mice with high (NOD SCID) and normal (C57Bl/6) radiosensitivity. * — significant differences between groups, р < 0.05
Fig. 5. Survival rate of the CD45lowCD34+ human cells in the model mice humanized with the peripheral blood HSCs as a function of the acute external gamma exposure dose. А. HSC survival rate three days after exposure. B. HSC number relative to the non-exposed control 14 days after exposure
Fig. 6. Relationship between the C14/3 coefficient and the dose for CD45lowCD34+ human cells in the model mice humanized with the peripheral blood HSCs isolated from adult humans