ISSN Print 2713-2757
ISSN Online 2713-2765
Extreme Medicine
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Assessing biodistribution of biomedical cellular product based on human chondrocytes following implantation to BALB/C nude mice
About authors
1 Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
2 Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia
3 Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
Correspondence should be addressed: Arina S. Pikina
Malaya Pirogovskaya, 1а, Moscow, 119435, Russia; ur.xednay@anikip.anira
About paper
Funding: the study was performed under the State Assignment “Chondrosphere”, R&D project ID АААА-А19-119052890054-4.
Acknowledgments: the authors express their gratitude to the research staff of the laboratory of cell biology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of FMBA of Russia, for methodological support provided during the study.
Author contribution: Pikina AS — literature review, literature source collection and analysis, manuscript writing; Golubinskaya PA — data acquisition and analysis, manuscript editing; Ruchko ES — data acquisition and analysis; Kozhenevskaya EV — carrying out work at the vivarium; Pospelov AD — histological analysis; Babayev AA — animal experiment management; Eremeev AV — experimental design, final correction of the manuscript. All authors confirm compliance of authorship to ICMJE international criteria.
Compliance with the ethical standards: the study was approved by the Boethics Commission of the Lobachevsky State University of Nizhny Novgorod (protocol № 73 dated 17 April 2023).
Received: 2023-11-05
Accepted: 2023-12-17
Published online: 2023-12-31
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Fig. 1. Changes in the experimental animals’ body weight by groups before and after the experiment. 1, 2 — groups of males and females, which underwent BCP implantation, n = 12; 3, 4 — groups of males and females, which received subcutaneous injection of saline, n = 12. * — significant intragroup differences, p < 0.05
Fig. 2. Chondropheres in murine tissues 90 days after implantation. Hematoxylin and eosin stain. 10х magnification. Scale bar — 100 µm
Table 1. Primers used in the study
Table 2. Human DNA detection
Note: Positive (indicative of the presence of human DNA) qualitative data were obtained for the samples designated as “+”; 1, 2 — groups that received BCP; 3, 4 — groups that received saline.
