ISSN Print 2713-2757
ISSN Online 2713-2765
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
Copyright: © 2023 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Assessing biodistribution of biomedical cellular product based on human chondrocytes following implantation to BALB/C nude mice
About authors
1 Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
2 Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia
3 Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
Correspondence should be addressed: Arina S. Pikina
Malaya Pirogovskaya, 1а, Moscow, 119435, Russia; ur.xednay@anikip.anira
About paper
Funding: the study was performed under the State Assignment “Chondrosphere”, R&D project ID АААА-А19-119052890054-4.
Acknowledgments: the authors express their gratitude to the research staff of the laboratory of cell biology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of FMBA of Russia, for methodological support provided during the study.
Author contribution: Pikina AS — literature review, literature source collection and analysis, manuscript writing; Golubinskaya PA — data acquisition and analysis, manuscript editing; Ruchko ES — data acquisition and analysis; Kozhenevskaya EV — carrying out work at the vivarium; Pospelov AD — histological analysis; Babayev AA — animal experiment management; Eremeev AV — experimental design, final correction of the manuscript. All authors confirm compliance of authorship to ICMJE international criteria.
Compliance with the ethical standards: the study was approved by the Boethics Commission of the Lobachevsky State University of Nizhny Novgorod (protocol № 73 dated 17 April 2023).
Received: 2023-11-05
Accepted: 2023-12-17
Published online: 2023-12-31
|
Fig. 1. Changes in the experimental animals’ body weight by groups before and after the experiment. 1, 2 — groups of males and females, which underwent BCP implantation, n = 12; 3, 4 — groups of males and females, which received subcutaneous injection of saline, n = 12. * — significant intragroup differences, p < 0.05
Fig. 2. Chondropheres in murine tissues 90 days after implantation. Hematoxylin and eosin stain. 10х magnification. Scale bar — 100 µm
Table 1. Primers used in the study
Table 2. Human DNA detection
Note: Positive (indicative of the presence of human DNA) qualitative data were obtained for the samples designated as “+”; 1, 2 — groups that received BCP; 3, 4 — groups that received saline.
