Local treatment of a contaminated skin wound using an original drug combination and magnetic therapy in an experiment

Terekhov AG, Pankrusheva TA, Chekmareva MS, Turenko EN, Artyushkova EB, Mishina ES, Grigoryan AYu, Myatechkin AA
About authors

Kursk State Medical University, Kursk, Russia

Correspondence should be addressed: Alexey G. Terekhov
Karla Marxa, 3, Kursk, 305041, Russia; ur.xednay@6464retxela

About paper

Author contribution: Terekhov AG — study concept and design, analysis of the resulting data, editing; Pankrusheva TA — design of the drug combination, data collection; Chekmareva MS — design of the drug combination, data collection; Turenko EN — collection of material, statistical data processing, analysis of the resulting data, article preparation; Artyushkova EB — collection of material, analysis of the resulting data; Mishina ES — collection of material, statistical data processing, analysis of the resulting data; Grigoryan AYu — analysis of the resulting data, article preparation, editing; Myatechkin AA — analysis of the resulting data, article preparation, editing.

Compliance with the ethical standards: the study was approved by the Ethics Committee of the Kursk State Medical University (Minutes #7 of November 30, 2020). The series of animal experiments, the conditions of their detention met the requirements of the Strasbourg Convention for the Protection of Animal Rights (France, 1986) and GOST 33044-2014 Principles of good laboratory practice.

Received: 2023-11-09 Accepted: 2023-12-19 Published online: 2023-12-31

Currently, treatment of contaminated skin wounds aggravated by ischemia of superficial soft tissues is a problem that presents certain difficulties. The search for the new ways of treatment and drugs possessing a multidirectional effect is a relevant problem. In this study, we aimed to explore the peculiarities of wound evolution and the effectiveness of the designed combination of medicines and magnetic therapy in a contaminated skin wound case. For the experiment, we divided male Wistar rats into 3 groups and modeled a contaminated skin wound in each of the animals. In the first group, no treatment was performed, in the second, we used the developed combination (benzalkonium chloride, dexpanthenol, pentoxifylline and carboxymethylcellulose sodium salt, combined with magnetic therapy), in the third — ointment with dioxomethyltetrahydropyrimidine + chloramphenicol and magnetic therapy. Planimetry, acid-base balance registration, measurements of microhemocirculation and local temperature of the wound bed underpinned monitoring assessment of the wounds. At the end of the study, the wound area in the second group was 10.7 and 3.7 (p < ; 0.05 ) times smaller than in the first and third groups, respectively, and healing rate — 2.6 and 1.3 (p < 0.05 ) times faster. The maximum values of microhemocirculation and the lowest pH were registered in the second group. Thus, combination of drugs and magnetotherapy we designed promoted healing of a contaminated skin wound, which allows recommending this treatment method for further study at the preclinical level.

Keywords: contaminated wound, local wound treatment, benzalkonium chloride, pentoxifylline, wound process