2023 / 04

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DOI: 10.47183/mes.2023.060

ORIGINAL RESEARCH

Isolation and characterization of virulent bacteriophages against Klebsiella pneumoniae of significant capsular types

Gorodnichev RB1, Kornienko MA1, Bespiatykh DA1, Malakhova MV1, Krivulia AO1, Veselovsky VA1, Bespyatykh JA1, Goloshchapov OV2, Chernenkaya TV3, Shitikov EA1
About authors

1 Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical Biological Agency, Moscow, Russia

2 Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russia

3 Sklifosovsky Research Institute for Emergency Medicine, Moscow, Russia

Correspondence should be addressed: Roman B. Gorodnichev
Malaya Pirogovskaya, 1а, Moscow, 119435, Russia; moc.liamg@b.r.vehcindorog

About paper

Funding: the study was conducted under the State Assignment “Development of a Complex Treatment Regimen for Drug-Resistant Pathogens Causing Infectious Diseases Using Bacteriophages and their Derivatives in Combination with Antimicrobial Drugs” (code: Bacteriophage-2). The Klebsiella pneumoniae strain typing was supported by the Russian Science Foundation grant (№ 22-15-00149, https://rscf.ru/project/22-15-00149/).

Acknowledgements: the whole-genome sequencing data were acquired using the equipment provided by the Core Facility Center “Genomics, Proteomics, Metabolomics” (http://rcpcm.org/?p=2806).

Author contribution: Gorodnichev RB — study plan, data acquisition and processing, manuscript writing; Kornienko MA — study plan, data acquisition and processing; Bespiatykh DA — data processing; Malakhova MV, Krivulia AO — data acquisition; Veselovsky VA, Goloshchapov OV, Chernenkaya TV, Bespyatykh YuA — data acquisition and processing; Shitikov EA — study plan, о data processing, manuscript writing.

Compliance with the ethical standards: experimental procedure was compliant with SanPiN 3.3686-21 “Sanitary Epidemiological Requirements for the Prevention of Infectious Diseases”; SanPiN 2.1.3684-21 “Sanitary and Epidemiological Requirements for the Maintenance of the Territories of Urban and Rural Settlements, for Water Bodies, Drinking Water and Drinking Water Supply, Atmospheric Air, Soils, Residential Premises, Operation of Industrial and Public Premises, Organization and Conduct of Sanitary and Anti-Epidemic (Preventive) Measures”, as well as Federal Clinical Guidelines "Rational Use of Bacteriophages in Clinical and Epidemiological Practice".

Received: 2023-11-01 Accepted: 2023-12-14 Published online: 2023-12-31
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