ISSN Print 2713-2757
ISSN Online 2713-2765
SCIENTIFIC AND PRACTICAL REVIEWED JOURNAL OF FMBA OF RUSSIA
ORIGINAL RESEARCH
Effect of sodium bicarbonate or hydrochloric acid intragastric administration on gut-derived endotoxemia in rats receiving cyclophosphamide myeloablative conditioning
About authors
1 Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency, Saint-Petersburg, Russia
2 State Scientific Research Test Institute of the Military Medicine of Defense Ministry of the Russian Federation, Saint-Petersburg, Russia
Correspondence should be addressed: Tatiana B. Pechurina
Lesoparkovaya, 4, Saint-Petersburg, 195043, Russia; ur.tsil@97tat
About paper
Author contribution: Vakunenkova OA — experimental part of the study; Zolotoverkhaya EA — blood biochemistry testing; Pechurina TB — tissue biochemistry testing; Schäfer TV — experimental part of the study, data processing and visualization, developing the experimental model; Ivnitsky JuJu — research design, developing the experimental model, data interpretation and discussion. All authors contributed to discussion, manuscript writing and editing.
Compliance with ethical standards: the study was compliant with the principles of bioethics adopted by the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes.
Received: 2024-05-01
Accepted: 2024-06-26
Published online: 2024-06-30
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Fig. 1. Mass indexes of gastric, caecal chyme, and spleen in rats 72 hours after intravenous administration of cyclophosphamide (M ± m; n= 8). Intact — rats that received no medicines; CP — rats that received only cyclophosphamide; NaHCO3 + CP + NaHCO3 — intragastric administration of 0.48 M of sodium bicarbonate 30 minutes before and immediately after cyclophosphamide; HCl + CP + HCl — intragastric administration of 0.1 M of hydrochloric acid 30 minutes before and immediately after cyclophosphamide. Significant difference, p < 0.05: * — with intact group; † — with the CP group
Fig. 2. Activity of alkaline phosphatase (left) and cholinesterase (right) in rats' small intestine tissues 72 hours after administration of cyclophosphamide (M ± m; n = 8). Significant difference, p < 0.05: * — with intact group; † — with the CP group
Fig. 3. Content of ammonia, urea, endotoxin, creatinine, albumin, and total protein in blood plasma sampled from rats' portal veins 72 hours after administration of cyclophosphamide (M ± m; n = 8). Significant difference, p < 0.05: * — with intact group; † — with the CP group
Fig. 4. Urinary excretion of indican, 48th to 72nd hours after administration of cyclophosphamide (M ± m; n = 8). * — significant difference with the intact group, p < 0.05
