Development of the COVID-19 Real-Time RT-PCR Testing System

Shuryaeva AK1, Malova TV1, Davydova EE2, Savochkina YuA1, Bogoslovskaya EV1, Mintaev RR1,2, Tsyganova GM1, Shivlyagina EE1, Ibragimova ASh1, Nosova AO1, Shipulin GA1, Yudin SM1
About authors

1 Federal State Budgetary Institution "Centre for Strategic Planning and Management of Biomedical Health Risks" of the Federal Medical Biological Agency, Moscow, Russia

2 I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

About paper

Funding: the study received funding from the Strategic Planning Center, Federal State Budgetary Institution under the Federal Medical-Biological Agency of Russia

Received: 2020-07-15 Accepted: 2020-08-13 Published online: 2020-08-19
Figure. Genome sequence alignment, coronaviruses SARS-CoV-2, OC43, HKU1, SARS, MERS, 229E and NL63, in the area of primer and probe design.
Table 1. Results of repeated clinical trials (assessment of diagnostic sensitivity and specificity) of the testing system
Table 2. Results of multiple alignment of 50386 known genome sequences of SARS-CoV-2 presented in the GISAID database, selected primers and probe regions. The columns indicate: 1 - sequence number of the nucleotide according to the MN985325 reference sequence, 2-5 identified polymorphisms among the analyzed sequences at this position, 6 - number of SARS-CoV-2 genome sequences in the GISAID database that do not differ at a given MN985325 position, 7 - number of sequences of SARS-CoV-2 GISAID genomes that differ in the given position, 8 - number of sequences of SARS-CoV-2 GISAID genomes that had no nucleotide established at the given position.